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. Author manuscript; available in PMC: 2016 Jan 1.
Published in final edited form as: Nat Immunol. 2015 May 18;16(7):766–774. doi: 10.1038/ni.3160

Figure 5. Cooperation between RAG1, RAG2 and AID promotes clonal evolution towards pre-B ALL.

Figure 5

(a) Immunoblot depicting protein expression of AID, RAG1 and RAG2 after retroviral overexpression of these vectors in EBV-immortalized human cord blood B cells. (b) Verification of Aicda (iRFP670), Rag1 (eGFP) and Rag2 (dsRedE2) overexpression in EBV transformed human B cells by flow cytometry (top) and verification of Rag1 and Rag2 overexpression in in these cells by fluorescence microscopy (bottom). (c) Whole exome sequencing analyses to compare the total counts of chromosomal abnormalities in empty vector (EV), Aicda, Rag1-Rag2, and Aicda Rag1-Rag2 transduced EBV-transformed human cord blood B cells.