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Genotyping of IVS9+459 T/C in FOXP3 gene using polymerase chain reaction-restriction fragment length polymorphism technique with TaaI (HPYCH4III) enzyme, followed by the band detection on gel red-stained 3% agarose gel after electrophoresis. 1: pUC19 marker, 2: TT in females and T in males (373 bp), 3: CT (just in females) (373,253, and 120 bp), 4: CC in females and C in males (253 and 120 bp)
