Figure 2.
Expression and purification of Tau 412 AA (htau 34); The samples were mixed with SDS- sample buffer, separated on 12% SDS-PEGE gel and stained with Coomassie Brilliant Blue R250. The tau monomer band is in the range of 48-63 kDa. A: Before IPTG- induction, B: After IPTG- induction, C: Mr. Marker D: Tau 412 AA (htau 34), E: tau after purification with Ni-NTA-Agarose precipitation