Figure 3.

Tau 412 AA (htau 34) was aggregated by arachidonic acid as an inducer of fibrillation for different time (0, 5, 24, 48, 72, 96 and 120 hr) which was measured by ThT emission spectrometry. Tau protein concentration was 20 μM, with 800 μM arachidonic acid in assembly buffer pH 7.4, and 3 mM DTT. The plateau was reached after 120 hr of incubation. A shift in the fluorescence emission spectra and enhancement of intensity were seen. Tau aggregation in the absence and presence of 100 μg/ml crocin (▲) and 100 μM methylene blue (○) was measured by ThT fluorescence spectrometry. Data are expressed as mean±SD, n=3. *** P<0.001 compared with tau group. MB and crocin are significantly different from tau (*** P<0.001)