Table 4.

Protein digestion analysis of final B-and T-cell epitopes

B-cell epitopes	Mass (Da)	pI	Undigested enzyme
30 TLGPKGRNVVL 40	1153.39	11.0	Chymotrypsin, Cyanogen Bromide, Iodobenzoate Benzoate, Staph Protease, AspN
150 ISANSDETVGKLIAEAM 166	1748.97	4.1	Chymotrypsin, Proline Endopeptidase, Cyanogen Bromide, Iodobenzoate Benzoate, Clostripain
219 ILLVDKKISNI 230	1255.56	8.6	Trypsin R, Proline Endopeptidase, Cyanogen Bromide, Clostripain, Iodobenzoate Benzoate, Staph Protease, Chymotrypsin
270 IVKVAAVKAP 279	995.27	10.0	Chymotrypsin, Proline Endopeptidase, Cyanogen Bromide, Iodobenzoate Benzoate, Clostripain, Trypsin R, AspN, Staph Protease
351 QQIEDSTSDY 360	1185.17	3.5	Trypsin, Chymotrypsin, Proline Endopeptidase, Cyanogen Bromide, Iodobenzoate Benzoate, Clostripain, Trypsin R, Staph Protease, Trypsin K
385 TEVEMKEKR 393	1149.33	5.9	Chymotrypsin, Proline Endopeptidase, Iodobenzoate Benzoate, Clostripain, Trypsin K
453 QIVENAGEEPSVVVNTVK 470	1912.13	4.2	Trypsin, Chymotrypsin, Cyanogen Bromide, Iodobenzoate Benzoate, Clostripain, Trypsin R, Trypsin K, AspN
526 DDKMDLGA 534	863.94	3.9	Chymotrypsin, Proline Endopeptidase, Iodose Benzoate, Clostripain, Trypsin R, Staph Protease

T-cell epitopes	Mass(Da)	pI	Undigested enzyme
160 KLIAEAM166	774.9	6	Chymotrypsin, Proline Endopeptidase, Cyanogen Bromide, Iodobenzoate Benzoate, Clostripain, Trypsin R, AspN
205 INKPETGSVELENPYILL 222	2029.32	4.25	Trypsin, Cyanogen Bromide, Iodobenzoate Benzoate, Clostripain, Trypsin R, Trypsin K, AspN
279 PGFGDRR 285	803.88	10.0	Cyanogen Bromide, Iodobenzoate Benzoate, Elastase, Trypsin K, AspN, Staph Protease
385 TEVEMKEKRAR 395	1376.59	8.26	Chymotrypsin, Proline Endopeptidase, Iodobenzoate Benzoate, Clostripain,Trypsin.
		9.8
418 ALVRVAAALTALT 430	1269.55	9.7	Chymotrypsin, Proline Endopeptidase, Cyanogen Bromide, Iodobenzoate Benzoate, Staph Protease, Trypsin K, AspN.
470 KGGKGNY 476	722.80		Chymotrypsin, Proline Endopeptidase, Cyanogen Bromide, Iodobenzoate Benzoate, Staph Protease, Trypsin R, Proline Endopeptidase, AspN, Elastase