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. Author manuscript; available in PMC: 2016 Jul 1.
Published in final edited form as: J Immunol. 2015 May 29;195(1):367–376. doi: 10.4049/jimmunol.1401607

Fig 6. Vaccination using mDCs is superior to pDCs in prolonging survival.

Fig 6

pDCs and mDCs isolated from spleen and cLN of WT CD45.2 mice, were stimulated overnight and loaded with SIINFEKL peptide. 5,000 antigen loaded DCs were used to vaccinate CD45.1 mice ic. injected with 2 × 105 GL261-OVA cells. A) DC distribution in various organs was analyzed at 1wpo. Host DCs and adoptively transferred DC were differentiated based on congenic marker CD45.1 and CD45.2. B) Percentage distribution of adoptively transferred pDCs and mDCs in brain and various lymphatic organs (n=6). C) Tumor bearing mice were vaccinated with pDCs or mDCs or PBS and followed for survival (n=10/group). * p<0.05, **p<0.01, ***p<0.001.