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. Author manuscript; available in PMC: 2016 Jul 1.
Published in final edited form as: J Immunol. 2015 May 20;195(1):185–193. doi: 10.4049/jimmunol.1403162

Figure 2. CD4-dependent recruitment of eosinophils to the lung at day 14 of Pneumocystis infection.

Figure 2

A. Bronchoalveolar lavage (BAL) of naïve (uninfected), wild type, and GK1.5 treated CD4-depleted mice 14 days post-inoculation with Pneumocystis shows a large population of cells with high side-scatter in wild type mice (left panel). The cells were gated as shown (left panel), and a SiglecF+CD11b+ population was seen in the wild type, but not the naïve or GK1.5 treated animals (right panel). B. Significant increase in percentage of SiglecF+CD11b+ cells in wild type animals compared to naïve and GK1.5 treated animals (n=4–5, **** p<0.0001 by one-way ANOVA with Tukey’s multiple comparisons). C. qRT-PCR for Epx (top) and Prg2 (bottom) on RNA extracted from BAL cell pellets shows significant increase in expression in wild type animals compared to naïve and GK1.5 treated animals (* p<0.05 by one-way ANOVA with Tukey’s multiple comparisons).