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. Author manuscript; available in PMC: 2016 Jul 1.
Published in final edited form as: J Immunol. 2015 May 20;195(1):185–193. doi: 10.4049/jimmunol.1403162

Figure 3. Eosinophils contribute to control of Pneumocystis infection both in vitro and in vivo.

Figure 3

A. BALB/c and Gata1tm6Sho/J knockout mice were infected with Pneumocystis and sacrificed at day 14 post-infection and SSU burden was quantified by qRT-PCR (** p<0.01 by student’s T test). Uninfected BALB/c mice have no detectable Pneumocystis burden. B. qRT-PCR for Epx (left) and Prg2 (right) on RNA from whole lung shows significant increase in BALB/c mice infected with Pneumocystis compared to uninfected BALB/c and infected Gata1tm6Sho/J knockout mice (* p<0.05 by Kruskal-Wallis test with Dunn’s multiple comparisons test). C. Bone marrow derived eosinophils from BALB/c mice demonstrate anti-Pneumocystis activity when co-cultured in vitro for 24 hours at an eosinophil to P. murina cyst ratio of 100:1 (*** p<0.0001, student’s t-test). D. Bone marrow derived eosinophils show enhanced killing activity when co-cultured with Pneumocystis in the presence of 10 ng/ml of IL-4 and IL-13 compared to Pneumocystis alone (** p<0.01 by student’s T test).