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. 2015 May 27;5:454–465. doi: 10.1016/j.fob.2015.05.005

Fig. 2.

Fig. 2

Immunofluorescent microscopy of chicken egg-coat matrix. Small pieces of the egg coat were stained with the mouse anti-tag (panels 1 and 3 of A) and anti-ZP1_repeat antisera (panels 2 and 4 of A), anti-ZP1_N-terminal, trefoil, ZP-N and ZP-C antisera (panels 1–4 of B, respectively), anti-ZP3_N-terminal, ZP-N and ZP-C antisera (panels 5–7 of B, respectively) and anti-ZPD_EGF, ZP-N and ZP-C antisera (panels 8–10 of B, respectively) being produced as described in Section 4 followed by Alexa Fluor® 488 goat anti-mouse IgG, and then observed under a confocal laser-scanning microscope equipped with a 488-nm excitation laser. Light images of differential-interference-contrast (DIC) microscopy are shown (panels 3 and 4 of A). Bar = 10 μm.