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Fibroblastic colony forming unit (CFU-F) assay for SVF recovered by aqueous phase separation as compared to whole digest centrifugation.
| Plating density | Centrifugation | Phase separation |
|---|---|---|
| (SVF cells/10 cm2) | ||
| 20000 | 72 ± 16 | 101 ± 24 |
| 10000 | 48 ± 14 | 42 ± 16 |
| 5000 | 20 ± 6 | 21 ± 5 |
Data represents mean number of CFU-F obtained for each plating density from three individual donors, each assayed in duplicate. Errors represent SD from three individual donor samples.
