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. 2015 Apr 15;156(7):2619–2631. doi: 10.1210/en.2014-1655

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This article has been published under the terms of the Creative Commons Attribution License (CC-BY; https://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Copyright for this article is retained by the author(s).

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Figure 3. — The effect of intra-ARC and intra-AVPV rAAV-kisspeptin-AS administration on estrous cyclicity in juvenile rats. Cyclicity was determined by analyzing daily vaginal cytology from day of vaginal opening to ovariectomy. A, ARC rAAV-kisspeptin-AS resulted in a significant decrease in percentage of normal estrous cyclicity vs rAAV-EGFP controls. Representative examples of estrous cyclicity are presented for ARC rAAV-EGFP (B) and ARC rAAV-kisspeptin-AS (C) rats. D, Cycle length was significantly extended after intra-ARC rAAV-kisspeptin-AS vs intra-ARC rAAV-EGFP controls. E, Time spent in proestrous, estrous, and metestrous stages was extended, whereas time spent in diestrus was decreased by ARC rAAV-kisspeptin-AS compared with controls. F, AVPV rAAV-kisspeptin-AS also resulted in a significant decrease in percentage of normal estrous cyclicity vs rAAV-EGFP controls. Representative examples of estrous cyclicity are presented for AVPV rAAV-EGFP (G) and AVPV rAAV-kisspeptin-AS (H) rats. I, Intra-AVPV rAAV-kisspeptin-AS also significantly extended the cycle length compared with intra-AVPV rAAV-EGFP. J, Intra-AVPV rAAV-kisspeptin-AS did not significantly extend the percentage of time spent in proestrus compared with AVPV rAAV-EGFP. Both estrous and metestrous cycle stages were prolonged after AVPV rAAV-kisspeptin-AS vs AVPV rAAV-EGFP. AVPV rAAV-kisspeptin-AS reduced time spent in diestrus. Results are presented as means ± SEM. *, P < .05 vs rAAV-EGFP controls (means ± SEM; n = 7–9 per group).