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Effects of BMP4 on SMAD 1/5/8 phosphorylation in H295R adrenocortical cells. Cells were incubated with BMP4 (30 ng/mL) for the indicated time points. The cells were then lysed and subjected to SDS-PAGE and immunoblotting analysis using an anti-pSMAD 1/5/8 antibody. Treatment of adrenocortical tumor cells with BMP4 shows integrity of BMP-dependent pathways by phosphorylation of SMAD 1/5/8. Results shown are representative of those obtained from three independent experiments.
