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. 2015 Mar 31;156(7):2552–2562. doi: 10.1210/en.2014-1974

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Figure 6. — Representative photomicrographs of Fos immunofluorescence in blank-SAP (A) and NK₃-SAP (B) rats injected subcutaneously with senktide 90 minutes before killing. After senktide injections, numerous Fos-ir cells were identified in the MnPO of control rats (A) but not in that of NK₃-SAP rats (B). C, Average numbers of Fos-ir cells counted in blank-SAP and NK₃-SAP rats injected with senktide. In the MnPO, the number of Fos-ir neurons was markedly decreased in NK₃-SAP rats, compared with those in controls. No significant differences were detected in other areas. D, Schematic diagram of landmarks at this level of the hypothalamus (43). Scale bar corresponds to 250 μm for A and B. Scale bar corresponds to 500 μm for D. Values represent means ± SEM; n = 8 rats/group. *, P < .05, significantly different, blank-SAP vs NK₃-SAP. ac, anterior commissure; AVPV, anteroventral periventricular nucleus; LPO, lateral preoptic area; MnPO, median preoptic nucleus; MPO, medial preoptic area; MS, medial septum; oc, optic chiasm; SHy, septohypothalamic nucleus; VLPO, ventrolateral preoptic area; 3V, third ventricle.