Table 1.

Excited state populations (p_E) and lifetimes (τ_E) for processes I–IV

DOI: http://dx.doi.org/10.7554/eLife.07296.014

Process	I	II	III	IV
pE (%)	3 ± 1	2.1 ± 0.1	3.3 ± 0.2	2.1 ± 0.1
τE (ms)	3.0 ± 0.6	13 ± 1	6.0 ± 0.6	1.6 ± 0.1

Uncertainties for process I are calculated as ±1 s.d. of the values obtained from single B₁ field fits of ¹⁵N CEST profiles of G51, N53, T54, and G61 (Figure 3—figure supplement 2B). Values for process II are the mean and standard deviation of p_E and τ_E obtained from the two best three-state models (Figure 3—figure supplement 2E). The equations for calculating these values are listed in the legend for Figure 3—figure supplement 2. Note that very similar values of p_E and τ_E are obtained from the two models (on- and off-pathway) and it is not possible to distinguish between them on the basis of the NMR data. Uncertainties for exchange parameters describing processes III and IV are obtained from global two-state fits of CPMG profiles reporting on each process. All values are relevant for a 1.3 mM protein concentration, 25°C.