FIG 6.

Chromosomal integration of flgE restored PF and motility. (A) PF of T. denticola were recovered after the integration of flgE into T. denticola genome. Electron microscopic examination of exposed PF (arrow) from spirochetal cells treated with 1% Triton X-100. Cells were stained with 2% uranyl acetate. (B) T. denticola colonies on agarose plates. Approximately 200 colonies after serial dilution were plated onto 0.8% agarose plates and incubated at 35°C for 5 days. (C) Measurement of representative colonies. Colonies were magnified, and their diameters were measured. Each bar represents 0.5 mm.