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. 2015 Jun 4;81(13):4316–4328. doi: 10.1128/AEM.00554-15

TABLE 1.

Mutant strains constructed and/or used in this study

A. vinelandii strain	Plasmid utilized	Genetic features^a	Parent strain
DJ^b	None	Wild type
AZBB010	pPCRNH3-15	ΔnifLA::Str^r	DJ
AZBB020	pPCRNH3-14	ΔnifLA::nifA	AZBB010
AZBB023	pPCRSCRK31	ΔscrX::lacZ-Kan^r	DJ
AZBB030	pPCRURE3	ΔureABC::Str^r	DJ
AZBB035	pPCRURE3	ΔureABC::Str^r and ΔnifLA::nifA	AZBB020
AZBB063	pPCRNH3-21	ΔnifLA::Tet^r and ΔscrX::lacZ-Kan^r	AZBB023
AZBB085	pEB001	amtB::Kan^r-transposon and ΔureABC::Str^r and ΔnifLA::nifA	AZBB035
AZBB086	pEB001	amtB::Kan^r-transposon and ΔureABC::Str^r and ΔnifLA::nifA	AZBB035
AZBB088	pEB001	amtB::Kan^r-transposon and ΔureABC::Str^r	AZBB030
AZBB091	pEB001	amtB::Kan^r-transposon and ΔureABC::Str^r	AZBB030
AZBB093	pEB001	amtB::Kan^r-transposon and ΔureABC::Str^r and ΔnifLA::nifA	AZBB035
AZBB094	pEB001	amtB::Kan^r-transposon and ΔureABC::Str^r and ΔnifLA::nifA	AZBB035
AZBB102	pPCRAMTBK3	ΔamtB::Kan^r	DJ
AZBB103	pPCRAMTBK3	ΔamtB::Kan^r and ΔureABC::Str^r	AZBB030
AZBB148	pPCRNH3-42	nifL::Kan^r similar to MV376^c	AZBB010
AZBB150	pPCRNH3-43	nifL::Kan^r incorporated slightly upstream of SmaI site	AZBB010
AZBB158	None	Spontaneous mutation of AZBB148 resulting in Nif⁺ phenotype	AZBB148
AZBB163	None	Spontaneous mutation of AZBB150 resulting in Nif⁺ phenotype	AZBB150

^a

Tet^r, tetracycline resistance; Str^r, streptomycin resistance; Kan^r, kanamycin resistance.

^b

Setubal et al. (35).

^c

Brewin et al. (8).