TABLE 3.
Primers used in this study
| Primer | Sequence (5′–3′)a | Purpose |
|---|---|---|
| BBP919 | GACTAGAA TTCGTGCAGA AACATCTCTA CCCGGAAG | ureABC gene and flanking region cloning |
| BBP920 | GACTAAAG CTTGGAACAG AAGACGATGA GGATGC | ureABC gene and flanking region cloning |
| BBP938 | GACTAGGA TCCGTGAAGA TCAGCAGCTT GTCTTTCTC | ureABC gene deletion |
| BBP939 | GACTAGGA TCCAAGAAGG ACCTGATCCA CAACG | ureABC gene deletion |
| BBP1147 | GAGCAAGC TTCATGGTCA GGTCGTGGCC TTC | nifLA gene and flanking region cloning |
| BBP1148 | GACAGGAT CCGGTCAGCA CTTCGGACAC CAC | nifLA gene and flanking region cloning |
| BBP1209 | GACATCTA GACTCTCATA TGGTGCCTCG TCTATCCAAG AAAACC | nifLA gene deletion |
| BBP1210 | GACATCTA GAGGATCCGA CCCTCCGGCA ATGGATG | nifLA gene deletion |
| BBP1149 | GACAGGAT CCATATGAAT GCAACCATCC CTCAGCGCT C | nifA gene cloning |
| BBP1150 | GACATCTA GACTAGATCT TGCGCATGTG GATGTTGAG | nifA gene cloning |
| BBP2099 | NNNGATAT CGGGCATTCC GCCCGACCTG GTGCTG | nifL gene segment deletion 1 |
| BBP2100 | NNNGATAT CGACGACGAT GCCCTGGGCC AGCAACTG | nifL gene segment deletion 1 |
| BBP2101 | NNNGATAT CCCGGAGAAG GCGCTGCCCT G | nifL gene segment deletion 2 |
| BBP2102 | NNNGATAT CCATCAGCAC CCGCGTGGAG AAC | nifL gene segment deletion 2 |
| BBP1674 | NNNTCTAG AGGAAGCTAT CCGACGAGGA CAGCCGAG | scrX gene and flanking region cloning |
| BBP1675 | NNNGAATT CCTGAGCGCA GAATTTAGAT ATTGATACTC ATAGTC | scrX gene and flanking region cloning |
| BBP1710 | NNNATGCA TATGGACTTC CTATTGTTGA CATTATTGGT GG | scrX gene deletion |
| BBP1711 | NNNATGCA TGACAGAATT CAGATCTCAC GCCATAAGCT GTTAGCATTT TTCTTG | scrX gene deletion |
| BBP1669 | NNNTCTAG AGGATCCCAT ATGCATACCA TGATTACGGA TTCACTGGCC GTCG | lacZ gene cloning |
| BBP1671 | NNNAAGCT TGGATCCTAT TTTTGACAC CAGACCAACT GGTAATGGTA G | lacZ gene cloning |
| BBP1967 | NNNAAGCT TCGAAGACAT GGCACTCCGA GGCGTTGGCC AGAC | amtB gene and flanking region cloning |
| BBP1968 | NNNTCTAG AGATAGATTC CCTGCCAGGT CCCCAG | amtB gene and flanking region cloning |
| BBP1969 | NNNAGATC TGGTTACAAC CTCTGAGTGT CGGGAG | amtB gene deletion |
| BBP1970 | NNNAGATC TCAGCGTCAT TGATTATTCT CCTGGGGCG | amtB gene deletion |
| BBP982 | GAAGGGCA GCAGCAGGTA GAGG | ureABC gene deletion confirmation |
| BBP983 | CAGCAGTT CGCGAAGACT GTCGAAG | ureABC gene deletion confirmation |
| BBP1836 | CTCAACGT TCGCCAGGTA TATGCCGAAC TC | scrX gene deletion confirmation |
| BBP1837 | CACATAGG ATGAAACGTC ACCGAGCTTG TTCG | scrX gene deletion confirmation |
| BBP950 | GAGCACAC CCATCACGGT CAGAG | nifLA gene deletion confirmation |
| BBP1322 | GATCTCCA TCGACTCGAT CTTGTCCAGG GTGAAC | nifLA gene deletion confirmation |
| BBP2006 | CACGTGCC AGGAATTCCT CCATG | amtB gene deletion confirmation |
| BBP2007 | CTGTGGAC GATGGCCAGG GACATGGATC | amtB gene deletion confirmation |
a
Specific restriction enzyme sites added to primers are underlined for clarity.