Monosaccharide and polyol concentrations in suspensions of ant-free fungus garden material incubated for 48 h at 25°C. Values are expressed per gram of fungus garden material dry mass. n = 12. Symbols: ■, whole; ●, whole plus NaN3; ▲, peripheral; ▼, peripheral plus NaN3; ◆, core; ◀, core plus NaN3. Sample size: four nests, one chamber per nest. Each point in the graph represents the results of four determinations.