FIG 1.

(A) Localization of Ssl2501-eGFP (GFP channel) in Synechocystis during exponential growth at an OD₇₅₀ of 0.8 and the autofluorescence of thylakoid membranes (Cy3 channel). (B) Synechocystis cells that had been nitrogen starved for 5 days and expressed Ssl2501-eGFP (GFP channel) were stained with Nile red (Cy3 channel) to visualize PHB. The yellow color in the overlay indicates a colocalization between Ssl2501-GFP and the Nile red-stained PHB granule. (C) Western blot detection of Ssl2501-eGFP during exponential growth at OD₇₅₀ of 0.8 and 0.4 using an anti-GFP primary antibody. (D) Western blot detection of Ssl2501-eGFP in nitrogen-starved cells using an anti-GFP primary antibody. Cell fracturing and separation in soluble and insoluble fractions were performed as described in Materials and Methods for the PHB synthase assay.