TABLE 1.
Frequency of bontE detection or quantification and average concentration at each sampling site and matrix
| Sampling site | Matrix | Total no. of samples | % of samples |
Avg no. of gene copies/mla (SD) | |
|---|---|---|---|---|---|
| Above LOQ | Above LOD | ||||
| Bay City | AlgOn | 11 | 63.6 | 90.9 | 99,662 (66,838) |
| Muck | 12 | 41.7 | 66.7 | 39,787 (16,738) | |
| SUB | 1 | 0 | 0 | Below LOQ | |
| SED | 9 | 22.2 | 22.2 | 8,335 (8) | |
| SOS | 8 | 12.5 | 37.5 | 12,227 (NA) | |
| SUM | 3 | 0 | 66.7 | Below LOQ | |
| Water | 8 | 100 | 100 | 1,511 (169) | |
| Portage | AlgOn | 7 | 42.9 | 100 | 35,487 (8,124) |
| SED | 10 | 30 | 70 | 19,260 (6,266) | |
| SOS | 10 | 10 | 30 | 14,897 (NA) | |
| Water | 10 | 80 | 100 | 1,900 (4,64) | |
| Jeorse | AlgOn | 12 | 66.7 | 83.3 | 71,941 (45,154) |
| SUB | 10 | 40 | 70 | 104,925 (53,158) | |
| Maumee | AlgOn | 19 | 10.5 | 52.6 | 25,602 (906) |
| SED | 12 | 41.7 | 41.7 | 21,861 (9,262) | |
| SOS | 7 | 28.6 | 28.6 | 21,346 (7,167) | |
| Water | 12 | 16.7 | 50 | 2,981 (1,250) | |
| SLBE | AlgOn | 10 | 10 | 40 | 4,725 (NA) |
| SED | 14 | 7.1 | 14.3 | 34,656 (NA) | |
| Water | 7 | 42.9 | 85.7 | 1,690 (150) | |
| Linwood | AlgOn | 11 | 0 | 0 | Below LOQ |
| SUB | 7 | 0 | 14.3 | Below LOQ | |
| Brimley | AlgOn | 4 | 0 | 75 | Below LOQ |
| Hammond | AlgOn | 12 | 0 | 16.7 | Below LOQ |
| SUB | 8 | 0 | 25 | Below LOQ | |
| Waukegan | AlgOn | 3 | 0 | 33.3 | Below LOQ |
| SUB | 1 | 0 | 0 | Below LOQ | |
| Deland | AlgOn | 12 | 0 | 0 | Below LOQ |
| SUB | 10 | 0 | 10 | Below LOQ | |
| Total | AlgOn | 101 | 20.8 | 46.5 | 68,359 (54,368) |
| SUB | 37 | 10.8 | 32.4 | 104,925 (53,137) | |
| Muck | 12 | 41.7 | 66.7 | 39,787 (16,738) | |
| SED | 45 | 24.4 | 35.5 | 19,855 (9,629) | |
| SOS | 25 | 16 | 32 | 17,454 (6,205) | |
| SUM | 4 | 0 | 50 | 0 | |
| Water | 37 | 56.8 | 81.1 | 1,825 (572) | |
a
Algae (AlgOn, muck, and SUB), gene copies/ml; sediment (SED, SOS, and SUM), gene copies/g (dry weight); water, gene copies/100 ml. NA, not applicable.