TABLE 1.
Major primers used in this study
| Primer | DNA sequence (5′–3′)a | Product size (bp) | Target gene or function |
|---|---|---|---|
| Cm_PacI_F | CCCTTAATTAATGCTCGGCGGTGTTCCTTT | 801 | Chloramphenicol resistance cassette |
| Cm_PacI_R | CCCTTAATTAAGCGCCCTTTAGTTCCTAAAG | ||
| CRISPR_F | CCACTTTTTCCACCCTTGAA | 1,718 | CRISPR locus |
| CRISPR_R | AGGGCTAGTGTTGATGATTGTG | ||
| TetOF | TTATTTTTGCATAAACAGATGATTAGTG | 2,308 | TetO from C. jejuni 81-176 |
| TetOR | GCAAGCTGTTAAGCTAACTTGT | ||
| Cj0031_F | ATTGAGGCTAAAAAGCCAAATTC | 1,588 | Cj0031 |
| Cj0031_R | GCTCTATCCAAAGCCTTAGCTTC | ||
| Cj0139_US_F | CTTATCCGTGATGGAGTTGTG | 1,000 | Cj0139 upstream 1 kb |
| Cj0139_US_R | GGCAAATTTCCCGGGTACCAAATCCTTAAATTATGTTATTTTTAA (SmaI) | ||
| Cj0140_DS_F | GATTTGGTACCCGGGAAATTTGCCTATCAAATGCACT (SmaI) | 1,000 | Cj0140 downstream 1 kb |
| Cj0140_DS_R | AAAGAAAAAGAAAAAAGTTCATTTTTAA | ||
| Cj0690c_US_F | TTACAAAAAAAAATAACACCACAAGAA | 1,000 | Cj0690c upstream 1 kb |
| Cj0690c_US_R | TAAATCTTTCATCCCGGGGGCTTTCTTTCTAAAGTATTTTTAAAAA (SmaI) | ||
| Cj0690c_DS_F | GAAAGAAAGCCCCCGGGATGAAAGATTTATATAGTTTTTAAAATTTAATTTT (SmaI) | 1,000 | Cj0690c downstream 1 kb |
| Cj0690c_DS_R | AGGAATTTTAGCAGATTTAAATGCG | ||
| HsdR_F | CAATAGCATGGCGGCTAAAT | 3,999 | HsdR |
| HsdR_R1 | TGGTGCAACTAAAATAGCAGGTT | ||
| Tet_Seq_R | CGGGACTGCTACTTTTTGTTC | Confirming insertional orientation | |
| Cm_Seq_F | TATTATGAGGAGGGCGGAAA | Confirming insertional orientation | |
| Cm_Seq_R | CAGGGCGTATTGCCAAAATA | Confirming insertional orientation |
a
Restriction sites are shown in boldface type and underlined in the primer sequence, and the restriction sites are identified in parentheses after the sequence.