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. 2015 Jun 4;81(13):4403–4410. doi: 10.1128/AEM.00463-15

TABLE 2.

Results of blaCMY-2 and blaCTX-M PCR of E. coli isolates from cattle feces in the Pacific Northwest

Time period Isolate selection or ceftiofur concn in MacConkey medium Total no. of isolates tested No. of farms blaCMY-2 positive
blaCTX-M positive
No. % (95% CI)a No. % (95% CI)a
2002-2003 Reduced susceptibility to ceftiofur per breakpoint agar test (17) 157 14 137 87.3 (80.8–91.9) 0 0 (0–3.0)
2008 Reduced susceptibility to ceftiofur per breakpoint agar test (17) 41 4 40 97.6 (85.6–99.9) 0 0 (0–10.7)
2008 8 μg/ml ceftiofur 24 4 23 95.8 (76.9–99.8) 0 0 (0–17.2)
2008 total 65 4 63 96.9 (88.4–99.5) 0 0 (0–7.0)
2011 0 μg/ml ceftiofur 45 4 14 31.1 (18.6–46.8) 2 4.4 (0.1–16.4)
2011 2 μg/ml ceftiofur 44 4 24 54.5 (39.0–69.3) 5 11.4 (3.0–20.2)
2011 total 89 4 38 42.7 (32.4–53.6) 7 7.9 (3.5–16.1)
2011 Reduced susceptibility to ceftiofur according to disk diffusion results (inhibition zone size of ≤22 mm) (19, 20) 47 4 37 78.7 (63.9–88.8) 7 14.9 (6.7–28.9)
a

CI, confidence interval (23).