FIG 3.

GCFs that likely produce two-component lanthipeptides. (A) A representative gene cluster of GCF146 and the sequence logos of the precursor peptide substrates. The predicted LanKC substrate/enzyme pair is shown in blue, and the predicted LanM substrate/enzyme pair is shown in red. The strictly conserved CxSxxS motif of the putative LanKC substrate and the GG/A leader peptide cleavage site of the putative LanM substrate are highlighted by red boxes. (B) A representative gene cluster of GCF30 that likely encodes a haloduracin-like system. The gene clusters appear in two different types, and homologous enzymes are linked by blue dashed lines. (C) A representative gene cluster of GCF46 that may encode class III two-component lanthipeptides. (D) A representative gene cluster of GCF140 that may encode three precursor peptides and a LanKC. The putative functions of the gene products are shown by colors. The logos of the precursor peptides in GCF30, GCF46, and GCF140 are shown in Fig. S2 to S4, respectively, in the supplemental material. The boundaries of each gene cluster are predicted based on the conserved genes within each GCF and are not clearly defined. RS, radical SAM; OR, oxidoreductase.