Table 2.
Localization of carbonylated proteins identified from old skeletal muscle biopsies.
| Protein name and localization | Swiss-Prot accession no | Protein spot noa | Mascot scoreb | Sequence coverage (%) | No. of sequenced peptides | Theoretical protein mass (kDa) | Theoretical PI | RMI ratioc |
|---|---|---|---|---|---|---|---|---|
| Ubiquitous proteins: | ||||||||
| Collagen alpha-1(VI) chain | CO6A1_HUMAN | 1 | 205 | 7 | 5 | 108 | 5.3 | 4.34 |
| Heat shock cognate 71 kDa protein | HSP7C_HUMAN | 5 | 256 | 15 | 5 | 71 | 5.4 | 1.63 |
| Glycerol-3-phosphate dehydrogenase [NAD+], cytoplasmic (GPD1) | GPDA_HUMAN | 13 | 214 | 22 | 4 | 38 | 5.8 | 1.52 |
| Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) | G3P_HUMAN | 14 | 243 | 12 | 3 | 36 | 8.6 | 1.67 |
| Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) | G3P_HUMAN | 15 | 424 | 25 | 5 | 36 | 8.6 | 1.68 |
| Voltage-dependent anion-selective channel protein 1 | VDAC1_HUMAN | 16 | 685 | 39 | 8 | 31 | 8.6 | 1.88 |
| Muscle specific proteins: | ||||||||
| Myosin-binding protein C, slow-type (MyBPC) | MYPC1_HUMAN | 2 | 119 | 3 | 3 | 128 | 5.8 | 1.54 |
| Glycogen phosphorylase, muscle form | PYGM_HUMAN | 3 | 882 | 22 | 13 | 97 | 6.6 | 1.67 |
| Myosin-7 | MYH7_HUMAN | 7 | 63 | – | 1 | 223 | 5.6 | 3.12 |
| Creatine kinase M-type | KCRM_HUMAN | 8 | 206 | 9 | 3 | 43 | 6.8 | 1.44 |
| Creatine kinase M-type | KCRM_HUMAN | 9 | 416 | 20 | 6 | 43 | 6.8 | 1.54 |
| Fructose-bisphosphate aldolase A | ALDOA_HUMAN | 10 | 611 | 31 | 7 | 39 | 8.3 | 1.65 |
| Troponin T, slow skeletal muscle | TNNT1_HUMAN | 11 | 512 | 22 | 6 | 33 | 5.9 | 1.87 |
| Troponin T, slow skeletal muscle | TNNT1_HUMAN | 12 | 244 | 22 | 5 | 33 | 5.9 | 1.42 |
| LIM domain-binding protein 3 (ZASP) | LDB3_HUMAN | 17 | 206 | 8 | 4 | 77 | 8.5 | 1.70 |
| Plasma proteins: | ||||||||
| Serotransferrin | TRFE_HUMAN | 4 | 399 | 17 | 10 | 77 | 6.8 | 1.31 |
| Serum albumin | ALBU_HUMAN | 6 | 986 | 29 | 13 | 69 | 5.9 | 1.33 |
Spots of interest were identified by MALDI-TO–FTOF-MS as described under Material and methods. For each spot, different parameters clarifying protein identification by MS are indicated.
a
Protein spot number refers to the numbered spots in Fig. 3.
b
Mascot protein scores greater than 56 are significant (P<0.05).
c
RMI ratio represents the Relative Modification Index ratio.