FIG 1.

Identification of miRNAs regulated by COX-2 in liver. (A) Identification of 30 differentially expressed miRNAs in Wt and hCOX-2-Tg liver, as detected by qPCR array. Values represent fold change relative to liver of Wt mice. (B to E) Validation of the upregulated expression of Let-7a and the downregulated expression of miR-23b, miR-146b, and miR-183 in Wt and hCOX-2-Tg liver, primary hepatocytes, and NCL-V (V) and NCL-C (C) and CHL-V (V) and CHL-C (C) cells by real-time PCR. miRNA expression was normalized to U6 RNA expression. Data are reported as means ± SD of the results of three independent experiments. *, P < 0.05 (versus absence of COX-2).