TABLE 1.

The oligonucleotide sequences

Gene	Orientation	Primer sequence (5′→3′)
Akt2	Forward	ACGTGGTGAATACATCAAGACC
	Reverse	GCTACAGAGAAATTGTTCAGGGG
Insig1	Forward	CTAGTGCTCTTCTCATTTGGCG
	Reverse	AGGGATACAGTAAACCGACAACA
Insig2	Forward	GGAGTCACCTCGGCCTAAAAA
	Reverse	CAAGTTCAACACTAATGCCAGGA
Irs1	Forward	CTCCTGCTAACATCCACCTTG
	Reverse	AGCTCGCTAACTGAGATAGTCAT
Irs2	Forward	ACCGACTTGGTCAGCGAAG
	Reverse	CACGAGCCCGTAGTTGTCAT
Prkca	Forward	AGAGGTGCCATGAGTTCGTTA
	Reverse	GGCTTCCGTATGTGTGGATTTT
Rplp0	Forward	ACTGGTCTAGGACCCGAGAAG
	Reverse	TCCCACCTTGTCTCCAGTCT
Tsc1	Forward	CAGCCGGTATTGCACATCCT
	Reverse	GGATAAACGGGTAGCAGCTTTG
Pck1	Forward	GGGCCGCTGGATGTCGGAAG
	Reverse	GGTGCGGCCTTTCATGCACC
Gck	Forward	TGAGCCGGATGCAGAAGGA
	Reverse	GCAACATCTTTACACTGGCCT
AKT2	Forward	ACCACAGTCATCGAGAGGACC
	Reverse	GGAGCCACACTTGTAGTCCA
INSIG1	Forward	GCCTACTGTACCCCTGTATCG
	Reverse	TGGTTAATGCCAACAAAAACTGC
INSIG2	Forward	TAATGCGGTGTGTAGCAGTCT
	Reverse	GTCCAATGGATAGTGCAGCCA
IRS1	Forward	CCCAGGACCCGCATTCAAA
	Reverse	GGCGGTAGATACCAATCAGGT
IRS2	Forward	CAGTGCTGAGCGTCTTCTTTT
	Reverse	ACCTACGCCAGCATTGACTT
PRKCA	Forward	ATGTCACAGTACGAGATGCAAAA
	Reverse	GCTTTCATTCTTGGGATCAGGAA
RPLP0	Forward	AGATGCAGCAGATCCGCAT
	Reverse	GTTCTTGCCCATCAGCACC
TSC1	Forward	GTGGCGGAAGTCTATCTCGTC
	Reverse	GCAAGGGTACATTCCATAAAGGC