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. 2015 Jun 19;35(14):2495–2502. doi: 10.1128/MCB.01453-14

FIG 5.

FIG 5

ArhGAP29 binds the PDZ-domain of Radil through its C terminus. (A) Domain architecture of Radil and the truncation mutants used for the assays depicted in panels B and C. RA, Ras association domain; DIL, dilute domain; PDZ, PSD-95/Dlg1/ZO-1. (B) Coimmunoprecipitation of V5-Radil truncation mutants with HA-ArhGAP29 expressed in HEK293T cells. The Western blot is representative of three independent experiments. (C) Live imaging of YFP-ArhGAP29 in HEK293T cells transfected with YFP-ArhGAP29 and HA-Rap1A(V12) in the presence of V5-Radil or V5-RadilΔPDZ. Experiments were repeated four times, and representative images were chosen. (D) Coimmunoprecipitation of V5-Radil with YFP-tagged full-length ArhGAP29 or ArhGAP29 lacking its last four amino acids (ArhGAP29ΔC) expressed in HEK293T. The Western blot is representative of two independent experiments. (E) Live imaging of YFP-ArhGAP29 in HEK293T cells transfected with YFP-ArhGAP29 or YFP-ArhGAP29ΔC with HA-Rap1A(V12) and V5-Radil. The experiments were repeated four times, and a representative image was chosen.