Fig. S4.

WT and IL-1R^−/− mice have similar numbers of injected alveolar macrophages, but IL-1R^−/− mice have fewer injected neutrophils during L. pneumophila infection. WT or IL-1R^−/− mice were infected with JR32 ΔflaA L. pneumophila intranasally. At 24 h PI, cells from the airway space and lung were isolated and loaded with CCF4-AM. The frequency and total number of injected airway alveolar macrophages (A), neutrophils (B), as well as pulmonary alveolar macrophages (C) and neutrophils (D) were quantified. Graphs show individual mice and mean from four pooled experiments. NS, not significant; **P < 0.005, ***P < 0.0005 as determined by Student’s t test.