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. 2015 May 15;5(5):e312. doi: 10.1038/bcj.2015.38

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Copyright © 2015 Macmillan Publishers Limited

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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Len in combination with SAHA triggers synergistic cytotoxicity. (a) MM.1S cells were cultured with Len (1.25–5 μm ) with vehicle control (□), and with 0.25 (), 0.5 () or 1 μm () SAHA for 48 h. Cell growth was assessed by MTT assay, and data represent mean±s.d. from three independent experiments. CI was calculated by CalcuSyn software program. (b–d) MM.1S cells were cultured with Len (2.5 μm) in the presence or absence of SAHA (0.5 μm) for 24 h. (b) Cells were harvested and subjected to annexin-V (X-axis)/PI (Y-axis) staining. Numbers indicate cell population in each quadrant. (c) Whole-cell lysates were subjected to immunoblotting with indicated Abs. (d) Cells were subjected to qRT-PCR analysis of c-Myc; 1, control; 2, Len 1.25 μm; 3, Len 2.5 μm; 4, Len 5 μm; 5, SAHA 0.25 μm; 6, SAHA 0.5 μm; 7, Len 2.5 μm+SAHA 0.25 μm; 8, Len 2.5 μm+SAHA 0.5 μm. Fold changes were normalized with internal control (GAPDH).

Inline graphic — Len in combination with SAHA triggers synergistic cytotoxicity. (a) MM.1S cells were cultured with Len (1.25–5 μm ) with vehicle control (□), and with 0.25 (), 0.5 () or 1 μm () SAHA for 48 h. Cell growth was assessed by MTT assay, and data represent mean±s.d. from three independent experiments. CI was calculated by CalcuSyn software program. (b–d) MM.1S cells were cultured with Len (2.5 μm) in the presence or absence of SAHA (0.5 μm) for 24 h. (b) Cells were harvested and subjected to annexin-V (X-axis)/PI (Y-axis) staining. Numbers indicate cell population in each quadrant. (c) Whole-cell lysates were subjected to immunoblotting with indicated Abs. (d) Cells were subjected to qRT-PCR analysis of c-Myc; 1, control; 2, Len 1.25 μm; 3, Len 2.5 μm; 4, Len 5 μm; 5, SAHA 0.25 μm; 6, SAHA 0.5 μm; 7, Len 2.5 μm+SAHA 0.25 μm; 8, Len 2.5 μm+SAHA 0.5 μm. Fold changes were normalized with internal control (GAPDH).