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. 2015 May 15;5(5):e312. doi: 10.1038/bcj.2015.38

Figure 4.

Figure 4

Len in combination with ACY1215 downregulates IKZF1/3. (a) H929 cells were transfected with scrambled (Sc) or HDAC6 targeted siRNA. Whole-cell lysates were subjected to immunoblotting with indicated Abs. (b) Transfected cells were also cultured with Len (0.3–3 μm) for 72 h. Cell growth was assessed by MTT assay. (c) MM.1S cells were cultured with Len (1 and 3 μm) for 48 h. Whole-cell lysates were subjected to immunoblotting. (d) MM.1S cells were cultured with ACY1215 (1 μm) for 16 h, and then treated with Len for 8 h (1 μm). Whole-cell lysates were subjected to immunoblotting with indicated Abs. (e) CD138-positive MM tumor cells from MM patients (n=7) were cultured with Len (2 μm) in the absence or presence of ACY1215 (2 μm) for 48 h. Cell growth was assessed by MTT assay. Data represent mean±s.d. from three independent experiments. *P<0.01; **P<0.02.