Figure 6.

Len with ACY1215 in combination with Dex or BTZ induces significant cytotoxicity. (a) MM.1S cells were cultured for 48 h with Len (1.25 and 2.5 μm) and ACY1215 (1–4 μm) in the absence or presence of Dex (50 nm). Cell growth was assessed by MTT assay. (b) MM.1S cells were cultured for 24 h with Len (1 μm) and ACY1215 (1 and 2 μm) in the absence or presence of Dex (50 nm). Whole-cell lysates were subjected to immunoblotting with indicated Abs. (c) MM.1S cells were cultured for 48 h with Len (1 μm) and ACY1215 (1 and 1.5 μm) in the absence or presence of BTZ (2–4 nm). Data represent mean±s.d. from three independent experiments.