Figure 5.
In vitro activity of 67-7A-mcMMAF and 7-1C-mcMMAF in tumor cell lines. Upon incubation of cells for 96 h with serial dilutions of 67-7A-mcMMAF (gray circle), 7-1C-mcMMAF (gray square), a nonspecific (ctrl) mouse IgG2a-mcMMAF (empty circle) or free MMAE (black diamond), cell viability was assessed by measurement of the ATP levels (Luminescence (CTG)). Mouse sarcoma cells transfected with SAIL (a), untransfected mouse sarcoma cells (b), OCI-AML3 (c and g), THP1 (d), NOMO1 (e) and KG1 (f). (g) Fluorescence intensity of SAIL in OCI-AML3 cells that were transduced by short hairpin RNAs (shRNAs) targeting SAIL compared with no shRNA transduced cells (left). In vitro activity of 7-1C-mcMMAF for OCI-AML3 cells with (shRNA pool 1) or without (shRNA pool 2 or no shRNA) SAIL knockdown (right). Results are the mean±s.e.m. of duplicate samples. The IC50 values and their associated 95% confidence intervals (95% CIs) are reported. NA, not applicable.