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. 2015 Jun 22;5:11198. doi: 10.1038/srep11198

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Copyright © 2015, Macmillan Publishers Limited

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Graphs represent cumulative data for 8 repeats. Using known quantities of wild type (WT) DNA (nil, 10 ng and 100 ng of DNA) against both mutant and WT probes for (a) ^V600EBRAF, (b) ^V600KBRAF and (c) ^Q61HNRAS mutations. There was no detectable mutant droplet count and the amount of droplets corresponding to WT DNA rose in proportion to the amount of DNA used. (NTC: no-template control. Total: total droplets generated.)