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. 2015 Jun 22;5:11398. doi: 10.1038/srep11398

Figure 6. Intracellular reactive oxygen species (ROS) are involved in the autophagy induced by CTAB-coated GNRs.

Figure 6

(a) HCT116 cells were treated with 0.25–4 nM CTAB-coated GNRs for 24 h, and the cells were then subjected to flow cytometry analysis of intracellular ROS. (b) Cells were treated with 2 nM CTAB-coated GNRs alone or in combination with 10 mM NAC for 24 h. Intracellular ROS levels were detected by flow cytometry. (c) Western blot analysis of the LC3 protein level. Cropping lines are used in the figure. Full-length blots are presented in Supplementary Figure 4. The gels have been run under the same experimental conditions. (d) Detection of acidic vesicular organelles with acridine orange staining.