Figure 4. The effects of depletion of clathrin on the internalization of M2 (del V³⁶¹-A³⁷³) and M2 (del K³⁷⁴-S³⁸⁰).

(A) Confocal micrographs show CCh-stimulated internalization of EGFP-tagged M2 WT, M2(del V³⁶¹-A³⁷³) and M2(del K³⁷⁴-S³⁸⁰) constructs, respectively. Cells were pre-treated with RFP-tagged vehicle or CHC shRNA for 72 h. (B) The extent of internalization was quantified for the constructs in A as described in ′′Methods′′. Scale bar, 10 μm. Mean ± SEM of at least 30 cells from at least three independent experiments are shown. ***p < 0.001.