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. 2015 Jun 22;5:11408. doi: 10.1038/srep11408

Figure 5. The sequences located in carboxyl-terminal domain of i3 determine the internalization dependence on clathrin for M4 and M2 mAChRs, respectively.

Figure 5

(A,B) Schematic representations of i3 loop of wild type and chimeric M2 (A) and M4 (B) mAChRs. The actual sequences of the analogous domains contained within the 20 amino acid for M2 receptor or 21 amino acid region for M4 receptor are shown. (C,E) Representative images of EGFP-tagged wild type and chimeric M2 (C) or M4 (E) mAChRs in response to CCh (60 min; 100 μM). Cells were transfected with RFP-tagged vehicle or CHC shRNA for 72 h. Scale bars, 10 μm. (D,F) The levels of internalization for M2-based (D) and M4-based (F) chimeric mAChRs in cells transfected with vector or shCHC were quantified. Mean ± SEM of at least 30 cells from at least three independent experiments are shown. *** p < 0.001.