Table 3.
Validation of differentially expressed genes related to isoflavonoid biosynthesis.
| Contig | Annotation | Fold change : RNA-Seq and qRT-PCR validation |
|||||||
|---|---|---|---|---|---|---|---|---|---|
| Lib 2/Lib 1 |
Lib 4/Lib 1 |
Lib 3/Lib 1 |
Lib 5/Lib 1 |
||||||
| RPKM | 2--ΔCt | RPKM | 2--ΔCt | RPKM | 2--ΔCt | RPKM | 2--ΔCt | ||
| 21904 | CHS | 0.49 | 1.87 | 48∗ | 36 | 0.76 | 0.97 | 0.47 | 0.60 |
| 14454 | CHI | 1.20 | 0.72 | 9.0 | 14.6 | 1.12 | 0.63 | 1.29 | 2.07 |
| 15184 | IFS | 20.1 | 2.90 | 25.1 | 6.84 | 3.31 | 2.45 | 2.69 | 2.14 |
| 01454 | HID | 5.87 | 7.86 | 2.27 | 12.3 | 4.04 | 8.51 | 6.07 | 25.1 |
Fold change of RPKM and 2-ΔCt between Lib 1 (Library 1) and other Libraries, respectively. ∗differently expressed gene detected by NOISeq-sim. CHS chalcone synthase, CHI Chalcone isomerase, IFS 2-hydroxyisoflavanone synthase, HID 2-hydroxyisoflavanone dehydratase.