Fig. 6.

Flies with FBit0019386 insertion showed sra upregulation. Real-time polymerase chain reaction quantification of sra and Bin1 transcript levels in outbred flies without FBti0019386 insertion and with FBti0019836 insertion. We represented the average expression level of sra (A and C) and Bin1 (B and D) relative to Act5C with SEM error bars for three biological replicates in 0–2 h embryos and in 5-day-old females. Normalized expression measured 2 h after chill-coma for sra and Bin1 is depicted in (C) and (D), respectively.