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. 2015 May 9;6:20. doi: 10.1186/s13227-015-0014-6

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© Salinas-Saavedra et al. 2015

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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In vivo cortical localization of NvPar-6::mVenus and NvLgl::mCherry during different stages of N. vectensis development. z-stack sections from the stages are shown in Figure 10. Beginning at later cleavage stages, both NvPar-6::mVenus (A) and NvLgl::mCherry (B) began to distribute in different regions inside the cells (C) below the cortical actin labeled with Lifeact::mTq2 (D): NvPar-6::mVenus was always observed towards the apical side while NvLgl::mCherry moved basolaterally in non-overlapping domains.