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. 2015 Jun 23;46(1):72. doi: 10.1186/s13567-015-0210-3

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© Nguyen et al. 2015

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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The efficiency of using ELISpot on enriched IgA ⁺ B-cell fractions to enumerate F4-specific IgA ⁺ antibody secreting cells (ASCs). A In one well, only one F4-specific IgA⁺ ASC was detected from 5 × 10⁵ peripheral blood monomorphonuclear cells (PBMCs) (left) while 18 F4-specific spots were detected from 5 × 10⁵ enriched IgA⁺ B-cells (right) at 21 days post primary immunization (dppi) of an oral immunized pig having maternal antibodies; each blue spot is counted as one F4-specific IgA⁺ ASC. B From eight wells, less than seven F4-specific IgA⁺ ASCs were detected from 4.0 × 10⁶ PBMCs whereas more than 28 F4-specific IgA⁺ ASCs were detected from 4.0 × 10⁶ enriched IgA⁺ B-cells at 21 dppi of the three immunized groups. The asterisk indicates a statistical significant difference (p < 0.05) between groups in comparison to the maternal-PBS group.