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. Author manuscript; available in PMC: 2015 Jun 22.
Published in final edited form as: Exp Biol Med (Maywood). 2014 Apr 15;239(9):1255–1263. doi: 10.1177/1535370214530369

Figure 5.

Figure 5

Expression of mesodermal genes after exposure to multiple, opposing gradients of ActivinA/BMP4 and SB-431542 or Dkk1. We quantified the expression levels of representative mesodermal and mesendodermal genes via qPCR. Data were obtained from ΔCt values and normalized to GAPDH. For each assay, 3–5 EBs per condition were pooled, from microwells in one row of the platform, corresponding to one single condition. Experiments without opposing inhibitors served as controls. hiPSC-derived EBs were subjected to a stable gradient of the chosen morphogens for 24 h between day 3 and day 4 after induction. Left inlet: Activin A (9 ng/ mL), BMP4 (13 ng/mL). Right inlet: SB-431542 (5 μM) for results in the left box, and Dkk1 (150 ng/mL) for results in the right box. Statistics were measured between control experiment and the corresponding inhibitor-based gradient for each microwell. *p <0.05, **p <0.005