Time course of Alexandrium excystment at 10, 12, 15, and 20 °C in the (a) light and (b) dark. Cysts were germinated in flasks using sediment collected from Bellingham Bay in February 2012. Percent excystment was determined by subtracting the number of cysts that remained in the flask (i.e., did not excyst) after 1, 3, 5, 7, 9, 14, 21, 42, and 63 days from the concentration of cysts at time zero. Markers represent the mean value of duplicate flasks for each time point.