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Effects of oncogenic RAS genes on RAS/MEK/ERK and PI3K/AKT/mTOR signaling of RMS13 cells. RMS13 cells expressing empty vector (EV), HRAS12V, KRAS12V, or NRAS12V were analyzed for RAS protein expression using a pan-RAS antibody (A), for expression and phosphorylation of ERK (B), and for expression and phosphorylation of AKT and S6 ribosomal protein (C) by Western blotting. Representative blots are shown.
