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Cell cycle analysis of GAS41 depleted HeLa H2B-GFP cells. The formation of a normal bipolar spindle (A), of a tripolar spindle (B), of a spindle with misaligned chromosomes (C), and of a spindle with a polarity switch to a bipolar metaphase after a tripolar prometaphase (D) are shown. The duration of metaphase to the next interphase in the different phenotypes is shown in (E).
