Figure 4. JMJD3 is involved in transcriptional regulation of genes identified by CHIP-Seq.

(a) Q-RTPCR analysis of RNA expression of 6 genes involved in NF-kB activation in OCI-AML3 cells after JMJD3 knock-down. (b) Immuno-histochemical analysis of phospho-p65 in the OCI-AML3 cells with JMJD3 knock-down (Top panel) and in control cells (Bottom panel). (c) H3K4me3 CHIP-PCR analysis of IL8RB, TYROBP, and FPR2 in the OCI-AML3 cells after JMJD3 knock-down. (d) H3K27me3 CHIP-PCR analysis of IL8RB in the OCI-AML3 cells after JMJD3 knock-down.