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. Author manuscript; available in PMC: 2015 Jun 22.
Published in final edited form as: J Proteome Res. 2015 Feb 27;14(4):1716–1726. doi: 10.1021/pr5010423

Figure 2.

Figure 2

Western blots and proteomics of CspA and SrfN levels from Salmonella WT and ΔrpoE strains in LB log, LPM 4h, and LPM 20h conditions. For Western blots (A, C), cspA or srfN gene was tagged with two HA at chromosomal level in WT and ΔrpoE strains. The same amount of cell lysates was loaded in each lane and probed for the indicated proteins and a control protein DnaK. For quantification, the ratio of CspA/DnaK or SrfN/DnaK was relativized to 100 in the WT background under LB condition. Proteomics data are represented by the log2 of peak areas ratios of (B) CspA and (D) SrfN in ΔrpoE divided by the WT strain.