TABLE III.
Quantitative autoradiography of [3H]mazindol binding in brains of wild-type and triple-opioid receptor knockout (KO) mice from the different genetic backgrounds
| [3H]mazindol-specific binding (fmol/mg tissue) | ||||||
|---|---|---|---|---|---|---|
| B6 | 129 | B6 × 129 | ||||
| Region | Wild-type | Triple KO | Wild-type | Triple KO | Wild-type | Triple KO |
| Nucleus accumbens core | 73.1 ± 13.9 | 52.4 ± 9.5 | 68.2 ± 14.2 | 59.0 ± 11.2 | 60.8 ± 11.6 | 46.8 ± 16.9 |
| Nucleus accumbens shell | 58.0 ± 12.3 | 37.9 ± 5.1 | 48.9 ± 8.1 | 44.6 ± 8.7 | 38.8 ± 12.5 | 38.2 ± 14.8 |
| Olfactory tubercle | 67.9 ± 12.1 | 93.9 ± 15.0 | 87.1 ± 8.2 | 81.9 ± 6.8 | 91.1 ± 8.5 | 76.1 ± 13.1 |
| Caudate putamen | 111.4 ± 19.8 | 88.7 ± 12.4 | 108.3 ± 17.1 | 87.0 ± 16.0 | 113.4 ± 22.2 | 77.9 ± 24.7 |
| Subthalamic area | 52.2 ± 12.4 | 59.2 ± 7.3 | 41.9 ± 7.8 | 48.2 ± 10.4 | 54.6 ± 15.0 | 42.1 ± 16.7 |
| Substantia nigra | 77.1 ± 13.8 | 79.0 ± 21.1 | 62.3 ± 15.2 | 49.8 ± 5.7 | 77.5 ± 10.0 | 84.3 ± 4.6 |
| Ventral tegmental area | 75.7 ± 15.0 | 77.2 ± 14.8 | 63.0 ± 19.3 | 54.6 ± 9.6 | 114.5 ± 12.0 | 99.4 ± 4.7 |
Quantitative autoradiography of [3H]mazindol binding in brains of wild-type and triple-opioid receptor knockout (KO) mice from different genetic background; C57BL/6 (B6), 129 (129), and C57BL/6 × 129 (B6 × 129). Values are expressed as means ± SEM of five to six mice. Three-way ANOVA showed there was a significant effect of region (F(6,182) = 12.85, P < 0.001) with no strain, genotype, strain × genotype interaction, or strain × genotype × region interaction effects.