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. Author manuscript; available in PMC: 2016 Jun 18.
Published in final edited form as: Mol Cell. 2015 Jun 4;58(6):989–1000. doi: 10.1016/j.molcel.2015.05.010

Figure 1. Amino acids promote the interaction of RagA with GATOR1 and RagA ubiquitination.

Figure 1

(A) Immunoprecipitation (IP) analysis of amino acid-dependent interaction between RagA and GATOR1 components (DEPDC5 and Nprl3) in HEK293T cells overexpressed with indicated proteins. Triangle indicates annotated protein. (B) IP analysis of amino acid-dependent interaction between endogenous RagA and GATOR1 components (DEPDC5 and Nprl3) in HEK293T cells. (C) IP analysis of amino acid-dependent RagA ubiquitination in HEK293T cells overexpressed with indicated proteins. (D) IP analysis of amino acid-induced ubiquitination of endogenous RagA in HEK293T cells. (E) IP analysis of RagC ubiquitination with or without amino acid stimulation in HEK293T cells overexpressed with indicated proteins. (F) IP analysis of amino acid-dependent K63-linked ubiquitination of endogenous RagA. Cells in (A–F) were starved for amino acids for 1 hour and restimulated with amino acids for the indicated time before lysis. See also Figure S1