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. Author manuscript; available in PMC: 2015 Jun 22.
Published in final edited form as: J Neurosci Res. 2010 Mar;88(4):746–757. doi: 10.1002/jnr.22253

Fig. 7.

Fig. 7

Induction of Birc3 expression is dependent on Fas expression. RT-qPCR results comparing Birc3 mRNA expression in FasL- treated wild-type (wt) vs. lpr (Fas-deficient) NPCs showed statistically significant Birc3 up-regulation in wt (**P < 0.01, n = 3) but not lpr NPCs. Both wt and lpr NPCs were isolated from 4-day postnatal BL6/C57 mouse pups according to the manufacturer’s instructions using NeuroCult Kit for CNS tissues. Cells were grown into free- floating neurospheres in complete medium before consequently disso- ciating into single cells and plating in a monolayer. These monolayer cells were grown to 80% confluence and treated with FasL for 48 hr before RNA extraction