TABLE I.
FasL Effects on Anti-apoptotic Gene ExpressionϮ
| Anti-apoptosis gene | Fold up- or down-regulation (FasL NPC/control NPC) |
|---|---|
| Akt1 | 1.07 |
| Api5 | 1.05 |
| Ataf | −1.19 |
| Bag1 | −1.15 |
| Bag3 | −1 |
| Bcl2 | 1.27 |
| Bcl2l1 | −1.15 |
| Bcl2l2 | −1.08 |
| Birc1a | 1.15 |
| Birc1b | 1.07 |
| Birc2 | 1.07 |
| Birc3 | 7.19** |
| Birc4 | 1.14 |
| Birc5 | 1.03 |
| Bnip3 | 1.05 |
| Casp2 | −1.25 |
| Cflar | 1.49 |
| Dad1 | 2.06* |
| Tsc22d3 | 1.01 |
| Hells | 1.52 |
| Il01 | 1.69 |
| Lhx4 | −1.29 |
| Mcl1 | −1.02 |
| Nfkb1 | 1.02 |
| Nme5 | 1.09 |
| Pax7 | 1.21 |
| Pim2 | 1.19 |
| Polb | 1.02 |
| Prdx2 | −1.15 |
| Rnf7 | −1.02 |
| Sphk2 | 1.01 |
| Tnf | −1.03 |
| Cd40lg | 1.08 |
| Zc3hc | 1.12 |
Ϯ
Results from mouse apoptosis gene array analysis. RNA was extracted from NPC control and FasL (200 ng/ml)-treated cells grown in minimal medium for 48 hr. Among all the antiapoptotic genes tested, Birc3 was the only one that showed a significant change in mRNA expression with FasL treatment. -Fold differences and P values obtained from averaging three separate arrays. Positive numbers indicate up-regulation; negative numbers indicate down-regulation. -Fold difference obtained with the equation (2−ΔCt FasL)/(2−ΔCt control)
*
P < 0.05
**
P < 0.00001